Search Application Screening Deals

Enzyme-based microtiter plate assay for @c-aminobutyric acid: App...

Category: (Book)

1 new, starting at $10.95

Buy Now

Editorial Review

This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
An enzyme-based microtiter plate assay for @c-aminobutyric acid (GABA) was developed. GABA was quantified using @c-aminobutyrate glutamate aminotransferase and succinic semialdehyde dehydrogenase in the presence of NADP^+ and @a-ketoglutarate. The NADPH produced by the series of enzymatic reactions was measured spectrophotometrically at 340nm. A linear relationship between absorbance and the concentration of GABA was obtained in the ranges from 5.0x10^-^4 to 1.0x10^-^2M. The relative standard deviation for 10 successive measurements was 0.9% at the 10mM GABA level. This analytical method was applied to the screening of GABA-producing lactic acid bacteria in de Man-Rogosa-Sharpe (MRS) medium. The proposed method enables one to assay 96 samples for an hour without the pre-treatment of samples. The method is by far superior to the traditional HPLC method from the point of view of rapidity and simplicity.